Part I. Investigations on Biofilmsin Health and Disease 1. Methods for Dynamic Investigations ofSurface Attached in vitro Bacterial and Fungal Biofilms
ClausSternberg, Thomas Bjarnsholt, and Mark Shirtliff 2. Aqueous Two-Phase System Technology forPatterning Bacterial Communities and Biofilms
Mohammed Dwidar, Shuichi Takayama, andRobert J. Mitchell 3. Quorum Sensing in Gram-Positive Bacteria:Assay Protocols for Staphylococcal agr andenterococcal fsr Systems
Akane Shojima and Jiro Nakayama 4. Advanced Techniques for in situ Analysisof the Biofilm Matrix (Structure, Composition,Dynamics) by Means of Laser Scanning Microscopy
Thomas R. Neu and John R. Lawrence 5. Multiplex Fluorescence in situHybridization (M-FISH) and Confocal Laser ScanningMicroscopy (CLSM) to Analyse Multispecies Oral Biofilms
Lamprini Karygianni, Elmar Hellwig, AliAl-Ahmad 6. Field Emission Scanning ElectronMicroscopy of Biofilm-Growing Bacteria Involved inNosocomial Infections
Claudia Vuotto and Gianfranco Donelli 7. Experimental Approaches to Investigatingthe Vaginal Biofilm Microbiome
Marc M. Baum, Manjula Gunawardana, and PaulWebster 8. Imaging Bacteria and Biofilms on Hardwareand Periprosthetic Tissue in Orthopaedic Infections
L. Nistico, L. Hall-Stoodley, and P. Stoodley 9. Animal Models to Evaluate BacterialBiofilm Development
Kim Thomsen, Hannah Trøstrup, and ClausMoser 10. Animal Models to Investigate FungalBiofilm Formation
Jyotsna Chandra, Eric Pearlman, and MahmoudA. Ghannoum 11. Non-Mammalian Model Systems toInvestigate Fungal Biofilms
Marios Arvanitis, Beth Burgwyn Fuchs, andEleftherios Mylonakis Part II. Investigations on anti-biofilm compounds andstrategies 12.Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors
Livia Leoni and Paolo Landini 13.In vitro Screening of Antifungal Compounds Able to Counteract BiofilmDevelopment
Marion Girardot and Christine Imbert 14.Biofilm Matrix-Degrading Enzymes
Jeffrey B. Kaplan 15.Efficacy Evaluation of AntimicrobialDrug-Releasing Polymer Matrices
IolandaFrancolini, Antonella Piozzi, and Gianfranco Donelli 16.Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy
Wean Sin Cheow and Kunn Hadinoto 17.Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections ofPseudomonas aeruginosa in vitro and in vivo
Wang Hengzhuang, Niels Høiby, and OanaCiofu 18.Contribution of Confocal Laser Scanning Microscopy in Deciphering BiofilmTridimensional Structure and Reactivity
Arnaud Bridier and Romain Briandet 19.Chip-Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibioticsand Biological Agents
Frida Mariana, Friederike Buchholz, andThomas Maskow 20.Bacteriophage Attack as an Anti-Biofilm Strategy
Sanna Sillankorva and Joana Azeredo 21.Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-BasedNosocomial Infections: Study Protocols
Francesco Giuliani Part III. Investigations on biofilms in theenvironment and manufacturing plants 22.Capturing Air-Water Interface Biofilms for Microscopy and Molecular Analysis
Margaret C. Henk 23.Biofilm-Growing Bacteria Involved in the Corrosion of Concrete WastewaterPipes: Protocols for Comparative Metagenomic Analyses
Vicente Gomez-Alvarez 24.Culture-Independent Methods to Study Subaerial Biofilm Growing onBiodeteriorated Surfaces of Cultural Heritage Stone Materials and Frescoes
Francesca Cappitelli, Federica Villa, andAndrea Polo 25.Biofilms of Thermophilic Bacilli Isolated from Dairy Processing Plants andEfficacy of Sanitizers
Sara A. Burgess, Denise Lindsay, and SteveH. Flint