题名 | Generation of mutant mice with minimal off-target effects by microinjection of one-cell embryos with paired sgRNAs and Cas9 nickase |
作者 | William C. Skarnes & Xingxu Huang |
链接 | http://www.nature.com/protocolexchange/protocols/2997#/anticipated_results |
http://www.nature.com/protocolex ... anticipated_results
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